Pluto Bioinformatics

GSE129826: Global analysis of enhancer targets: Mosaic-seq

Bulk RNA sequencing

Single-cell perturbation assays such as Mosaic-seq enable highly multiplexed functional assessment of enhancers in their endogenous genomic context. By introducing a few computational and experimental improvements, we expanded the Mosaic-seq analysis to capture the secondary gene targets of enhancers. Our analysis of >500 putative enhancers in K562 cells demonstrates that many secondary hits are shared among enhancers targeting different transcriptional factors, which reveals an interwoven enhancer-driven gene regulatory network. Together, our data underscore the flexibility of manipulating gene transcription by modifying enhancer activity. SOURCE: Gary Hon (Gary.Hon@UTSouthwestern.edu) - UT Southwestern Medical Center