Gata3 is a DNA-binding transcription factor involved in cellular differentiation in a variety of tissues. To investigate the role of Gata3 in lens development we conducted lens-specific conditional loss-of-function model of Gata3 and confirmed abnormal regulation of cell cycle exit-coupled lens differentiation and retention of nuclei in the mutated lens fibers. We analyzed transcriptomes between control and mutated lenses by RNA-seq and revealed dysregulation of genes encoding critical components of the lens differentiation cascade, including a subset of crystallin genes, down-regulation of Dnase2b and reduced expression of Cdkn1b/p27 and Cdkn1c/p57. SOURCE: Ales Cvekl (ales.cvekl@einstein.yu.edu) - Dr. Cvekl's lab Albert Einstein College of Medicine

Pluto Bioinformatics

GSE131291: Gata3 directly regulates cell cycle exit-coupled lens fiber cell differentiation in mouse embryonic lens