Pluto Bioinformatics

GSE103383: RNA Seq analysis of pancreatic beta cell transcriptome during dedifferentiation

Bulk RNA sequencing

We report RNA Seq analysis using Illumina nextSeq500 of human beta cells EndoC-BH1 treated with FGF2 to induce dedifferentiation.; FGF2 treatment induced dedifferentiation of EndoC-BH1 cells. Indeed, we observed a strong decrease in expression of -cell markers, (INS, MAFB, SLC2A2, SLC30A8 and GCK). Opposingly, we identifed positive markers of human cell dedifferentiation, as attested by increased expression of mature -cell disallowed transcription factors (MYC, HES1, SOX9 and NEUROG3). Interestingly, our temporal analysis revealed that loss of expression of cell specific markers preceded the induction of cell disallowed genes. SOURCE: Fabrice CHIMIENTI (fabrice.chimienti@gmail.com) - AstraZeneca