Pluto Bioinformatics

GSE62889: Genome-wide expression profile of the Tet-On HCT116 inducible cell line that express either the human HNF42 or HNF48 under control of Doxycycline (DOX) [RNAseq]

Bulk RNA sequencing

Purpose: Aim of the study is to identify functional differences between the P1 and P2-HNF4 isoforms. To do this, we generated Tet-On inducible lines that express either the human (P1) HNF42 or (P2) HNF48 under control of DOX in the HCT116 human colon cancer cells. Methods: HNF42 and Parental lines were induced with 0.3 g/mL DOX, while HNF48 line was induced with either 0.1 or 0.3 g/mL DOX for 24 hours. Samples were generated by deep sequencing, using the Illumina TruSeq RNA. Result: There were common and unique dysregulated genes identified in the HNF42 and HNF48 lines (+DOX); more upregulated genes than downregulated genes in both the lines. Conclusion: The functional difference between HNF42 and HNF48 is that the latter tends to upregulate genes involved in proliferation and anti-apoptosis while HNF42 upregulates genes involved in growth suppression and cell death. SOURCE: joseph,M,dhahbi (jdhahbi@ucr.edu) - Biochemistry University of California, Riverside