Pluto Bioinformatics

GSE111417: RNAseq of germinal center B cells from Blimp-1-GFP reporter mice with an ablatable TfH compartment.

Bulk RNA sequencing

RNAseq of germinal center B cells (B220+IgDlowCD95+GL7+) with different Blimp-1-GFP reporter level in the presence of TfH cells or 72 hr after their depletion. Sorted germinal center populations were defined by their expression of Blimp-1-GFP reporter and the cell surface marker CD138. Analysed mice were bone marrow chimeras generated with a bone marrow mixture from CD4-cre+ Rosa26-loxP-stp-loxP-DTR Blimp-1GFP/+ (10%) and Rag1-/- OTII (90%) mice. In these mice TfH cells can be depleted by the injection of human diphteria toxin (DT). SOURCE: Daniel RadtkeOliver Bannard Laboratory Weatheral Institute of Molecular Medicine