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GSE61517: Strand-specific Dual RNA-seq of Bronchial Epithelial cells Infected with Influenza A/H3N2 Viruses Reveals Splicing of Gene Segment 6 and Novel Host-Virus Interactions

Bulk RNA sequencing

We employed massively parallel RNA sequencing to explore host and virus transcriptome and potential alternative splicing events occurring during host-virus interplay. Here, we employed a panel of two seasonal influenza A (H3N2) strains that circulated in the human population and are equipped with an intact NS1 antagonist (BR10/07 and PER16/09), and the laboratory adapted strain A/Udorn/307/72 (H3N2). By infecting the bronchial epithelial cell line BEAS-2B, we determined similarities and differences in the spectrum and magnitude of cytokines and chemokines to distinct viral strains. Given the clinical importance of IAV/H3N2, we used strand-specific RNA sequencing (RNA-seq) to characterize virus-host interactions and to explore differences between the seasonal and laboratory IAV/H3N2 strains. In addition to global gene expression changes, we identified novel alternative splicing (AS) events of the viral neuraminidase gene and of host transcripts, which in turn may modulate innate immune cellular pathways. Our findings highlight the importance of studying innate antiviral host responses to clinically relevant strains and illustrate novel molecular events occurring during host-influenza virus interactions. SOURCE: Andrew,J,Oler (andrew.oler@nih.gov) - Computational Biology Section NIAID/NIH

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