Pluto Bioinformatics

GSE112920: IL-27 in vaccine elicited cellular immunity

Bulk RNA sequencing

5000 congenically marked (CD45.1) WT or IL-27R-/- OT1 T cells were transferred into WT B6 hosts (CD45.2). One day later, the mice were immunized with ovalbumin (100 g), PolyIC (40 g) and anti-CD40 antibody (FGK4.5, 40 g). 3 days after immunizayion, spleens were harvested and the OT1 T cells isolated by flow sorting based on CD45.1 expression. Each sample is the result of pooling T cells from 3 individual mice. RNA was isolated from the sorted cells and RNAseq performed. SOURCE: Ross Kedl (ross.kedl@ucdenver.edu) - University of Colorado Anschutz Medical Campus