The goal of this analysis is to profile AR-regulated genes, especially non-coding RNAs in three androgen sensitive prostate cancer cell lines, MDA-PCA-2B, LNCaP and VCaP. The two cell lines were serum-starved first, followed by dihydrotestosterone (DHT) stimulation or treated with Enzalutamide (AR inhibitor) without starvation. Transcriptome profiling was generated by RNA-sequencing from polyA-selected RNA. These experiments are followed by knock-down experiments of AR and ARlnc1 in MDA-PCA-2B, and also Enzalutamide (anti-androgen) treatment of LNCaP cells. SOURCE: Marcin,Piotr,Cieslik (marcin.cieslik@gmail.com) - MCTP University of Michigan

Pluto Bioinformatics

GSE110903: RNA-seq profiling identifies Androgen Receptor-regulated genes in prostate cancer cells