Pluto Bioinformatics

GSE115813: Metabolic labeling of Hek293 cells using 4-thiouracil

Bulk RNA sequencing

Hek293 cells were metabolically labelled using 4-thiouracil as described in (Schwalb et al, Science. 2016 Jun 3;352(6290):1225-8) but without fragmentation, and then bulk RNA was prepared for sequencing using the STRT method (Islam et al, Genome Res. 2011 Jul;21(7):1160-7). Samples were incubated in duplicate for 5, 15 and 30 minutes and included an unlabeled control representing the steady-state expression state. SOURCE: Sten LinnarssonMolecular Neurobiology Karolinska Institutet