Pluto Bioinformatics

GSE97036: Effects of arsenic and Pseudomonas aeruginosa infection on gene expression in human airway epithelial cells

Bulk RNA sequencing

Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection; Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR; Findings: 20-30 million reads were mapped per sample and transcripts were identifed that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa SOURCE: Britton,C,Goodale ( - Stanton Geisel School of Medicine at Dartmouth

View this experiment on Pluto Bioinformatics