Pluto Bioinformatics

GSE139239: RNA sequecing of E0771 DDR1 WT/KO mammary tumor transplanted from Rag1-/- to C57BL/6 hosts

Bulk RNA sequencing

Purpose: The goals of this study are to compare mammary tumor transcriptome profiling with or without DDR1 in immunocompetent mice in an unbiased way.; Methods: E0771 DDR1 WT or KO tumor cells are firstly inoculated into Rag1-/- mice. When tumor volume reached approximately 200~300 mm3 (usually 20 days after inoculation), 60 mg of tumor organoid were transplanted to WT C57BL/6 mice. Tumor samples were collected on day 12 for RNA-seq, using Illumina HiSeq 3000 platform. 50 bp single read sequencing was conducted.; Results: Approximately 50 million sequence reads were obtained per sample and identified more than 20,000 transcript isoforms.; Conclusions: The RNA-seq results represents the detailed analysis of DDR1 WT/KO mammary tumor transcriptomes in immunocompetent mice C57BL/6. SOURCE: Xiujie Sun ( - The George Washington University

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