Pluto Bioinformatics

GSE157215: Brain RNA-seq in the LXS RI strains: Saline and ethanol treatment

Bulk RNA sequencing

Purpose: To investigate the effect that acute ethanol tretament (5 g/kg) has on the brain transcriptome and relate that to ethanol-related behaviors.; Methods: Male LXS recombinant inbred mice (40 strains; n=2 or 3 per strain) were treated with saline or ethanol (i.p.; 5 g/kg). Paired-end RNA-seq methods were employed to assess mRNA expression in whole brain 8 hours after the treatment.; Results: The expression of thousands of transcripts was altered by the ethanol treatment. Thousands of expression QTLs (eQTLs) were mapped. A large percentage of the eQTLs were unique to the treatment. SOURCE: Richard,A,Radcliffe (richard.radcliffe@cuanschutz.edu) - University of Colorado Anschutz Medical Campus