Pluto Bioinformatics

GSE109185: Transcriptome analysis of iPSC-derived keratinocytes with or without a disease-associated TP63 mutation

Bulk RNA sequencing

Induced pluripotent stem cells (iPSC) were generated from two patients affected by ankyloblepharon ectodermal dysplasia and clefting (AEC), an ectodermal dysplasia caused by mutations in TP63. The two TP63mutations(I537T and R598L) were corrected using Crispr/Cas9- mediated homologous recombination. The resulting conisogenic iPSC pairs were differentiated into keratinocytes and subjected to RNA-sequencing. SOURCE: Maranke Koster (Maranke.koster@ucdenver.edu) - University of Colorado-Anschutz Medical Campus