Pluto Bioinformatics

GSE123523: Comparing transcriptomic analysis using QuantSeq and RNA-Seq with Salmon quantification and Tophat2 mapping

Bulk RNA sequencing

We have compared a relatively new method for RNA 3 prime sequencing, QuantSeq with traditional RNA-Seq which captures reads from the entire RNA-transcript. In addition, we have compared read quantification using traditional base-to-base mapping (Tophat2) with the new pseudoalignment method Salmon. We have performed this experiment on peripheral blood mononuclear cells (PBMC) stimulated with Poly (I:C), a viral mimic that induces innate antiviral responses. SOURCE: Susan Corley (s.corley@unsw.edu.au) - Systems Biology Initiative UNSW