Pluto Bioinformatics

GSE124578: RNA-seq on Fixed and Permeabilized Plasma cells

Bulk RNA sequencing

Here we developed a protocol to extract total RNA from fixed and permeabilized cells that were intracellularly stained and FACS isolated. We applied these techniques to derive the transcriptome of influenza- and BCR isotype-specific antibody secreting cells or plasma cells. These data reveal that ASC isotypes have more distinct transcriptional signatures than previously appreciated. SOURCE: Chris Scharer (cdschar@emory.edu) - Jeremy Boss Emory University

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