Pluto Biosciences, Inc

GSE151824: Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation

Bulk RNA sequencing

Anterior mesoderm (AM) and definitive endoderm (DE) progenitors represent the earliest embryonic cell types that are specified during germ layer formation at the primitive streak (PS) of the mouse embryo. Genetic experiments indicate that both lineages segregate from Eomes-expressing progenitors in response to different NODAL signaling levels. However, the precise spatiotemporal pattern of these emerging cell types and molecular details of lineage segregation remain unexplored. We combined genetic fate labeling and imaging approaches with scRNA-seq to define transcriptional identities and lineage dynamics of Eomes-dependent cell types. Here we show that all cells moving through the PS during the first day of gastrulation express Eomes. AM and DE specification occurs before cells leave the PS from discrete progenitor populations that are generated in distinct spatiotemporal patterns. Importantly, we dont find evidence for the existence of progenitors that co-express markers of both cell lineages suggesting an immediate and complete separation of AM and DE lineages. SOURCE: Sagar - (sagar@ie-freiburg.mpg.de) - Dominic Grün Max Planck Institute Freiburg

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