Pluto Bioinformatics

GSE130910: To investigate whether recover DNMT3A expression permitted adult beta cell of RapKO establishing proper transcriptome

Bulk RNA sequencing

Murine islets were isolated from 8-week-old WT and RapKO mice and initially recovered in 1640 RPMI supplemented with 10% serum, 11.1mM glucose overnight. Then islets were transfected with Lenti-GFP or Lenti-Dnmt3a for 24 hours, following cultured in 1640 RPMI medium for 48 hours.Islets were harvested and stored in -80C for preparation for RNA sequencing. SOURCE: Qicheng Ni (niqicheng8@qq.com) - Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine