The goal of this study are to find the abonormally expressed genes of  B7-H4-/- leukemia inital cells in MLL-AF9 induced AML mice by RNA-seq analysis.Methods:  Lin- festival liver cells were isolated from WT and B7-H4-null mice and infected with MLL-AF9 retroviruses, then transplanted to the lethally  irradiated recipient mice. When AML developed, we isolated the  YFP+/Mac-1+/c-Kit+  bone marrow cells  by FACS and and then  made the RNA-sequence using Illumina. Results:  We  identified 27180 transcripts in wild type and B7-H4-/- leukemia initial cells. 572 transcripts showed differential expression between the WT and B7-H4/ LICs, with a fold change 1.5 and p value <0.01. 271  transcripts were downregulated and 241 transcripts were upregulated. SOURCE: Fangzhen Xia (xiafangzhen@126.com) - Key  Laboratory  of  Cell  Differentiation  and  Apoptosis  of  Chinese  Ministry  of  Education Shanghai  Jiao  Tong  University  School  of  Medicine

Pluto Bioinformatics

GSE89161: Analysis of Wild Type and B7-H4-/- leukemia initial cells Transcriptomes