Pluto Bioinformatics

GSE111168: Next Generation Sequencing Facilitates Quantitative Analysis of Retinoblastoma Transcriptomes

Bulk RNA sequencing

Methods: mRNA profiles of retinoblastoma samples and para-tumor were generated by deep sequencing, in triplicate, using Illumina. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: BurrowsWheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRTPCR validation was performed using TaqMan and SYBR Green assays SOURCE: Peiwei Chai (chaipeiwei123@sjtu.edu.cn) - Shanghai Ninth people's hospital