Changes in DNA methylation are required for the formation of germinal centers (GC), but the mechanisms of such changes are poorly understood.  Activation-induced cytidine deaminase  (AID) has been recently implicated recently in DNA demethylation through its deaminase activity coupled with DNA repair.  We investigated the epigenetic function of AID in vivo in germinal center B cells (GCB) isolated from wild type (WT) and AID-deficient (Aicda-/-) mice.  We determined that the transit of B cells through the GC is associated with marked locus-specific loss of methylation and increased methylation diversity, both of which are lost in Aicda-/- animals.  Differentially methylated cytosines (DMCs) between GCB and nave B cells (NB) are enriched in genes that are targeted for somatic hypermutation (SHM) by AID and these genes form networks required for B cell development and proliferation. Finally, we observed significant conservation of AID-dependent epigenetic reprogramming between mouse and human B cells. SOURCE: Matt Teater (mrt2001@med.cornell.edu) -  Weill Cornell Medical College

Pluto Bioinformatics

GSE71702: DNA Methylation Dynamics of Germinal Center B Cells Are Mediated by AID