Pluto Bioinformatics

GSE100277: Next-generation sequencing measuring mRNA expression in nave T cells isolated from Mettl3 KO and WT mice

Bulk RNA sequencing

Nave T cells were obtained by StemCell CD4+ T cell isolation kit of spleen from Mettl3 KO and WT mice followed by FACS sorting (CD4+CD25-CD45RB-Hi). Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat, and gene expression levels were measured by Cufflinks. SOURCE: Huabing Li (huabing.li@yale.edu) - Yale University School of Medicine