Pluto Bioinformatics

GSE78779: CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Bulk RNA sequencing

Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with three-fold higher sensitivity, lower costs, and less hands-on time. We also implemented CEL-Seq2 on Fluidigms C1 system, thereby providing its first single-cell, on-chip barcoding method, and detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2s increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use SOURCE: Naftalie Senderovich (naftalys@tx.technion.ac.il) - Itai Yanai Technion - Israel Institute of Technology