Pluto Bioinformatics

GSE90697: Organism-level analysis of vaccination reveals networks of protection across tissues

Bulk RNA sequencing

To study the response of single lung and liver memory CD8+ T cells to reactivation, mice vaccinated subcutaneously with MVA (10^7 PFUs/mouse) were subsequently challenged intranasally with: (1) PBS; (2) WR (10^7 PFUs/mouse); or (3) Vaccinia virus-derived B8R20-27 peptide (20 g/mouse). Sixteen hours after challenge, single-cell suspensions were generated from lung or liver and lymphocytes were enriched using Percoll density centrifugation prior to immunostaining and cell sorting. Single memory CD8+ T cells from lung and liver were profiled using plate-based or droplet-based single-cell RNA-seq. SOURCE: Nicolas Chevrier (nchevrier@uchicago.edu) - Nicolas Chevrier The University of Chicago