Pluto Bioinformatics

GSE115526: Molecular profiling of reticular gigantocellularis neurons

Bulk RNA sequencing

we used technique that allows the molecular characterization of particular neuronal subpopulations based on their neuroanatomical projections and the locations of their cell bodies. This retro-TRAP (translating ribosome affinity purification from retrogradely labeled neurons) approach relies on viral injection into an anatomical area targeted by the neurons of interest, followed by selective precipitation of ribosomes from retrogradely labeled cell bodies, and subsequent RNAseq analysis. SOURCE: Inna Tabansky (tabansky@post.harvard.edu) - Pfaff The Rockefeller University