Derivation of human nave cells in the ground state of pluripotency provides promising avenues for developmental studies and therapeutic manipulations. However, the molecular mechanisms involved in establishment and maintenance of human nave pluripotency remain poorly understood. Using the human inducible reprogramming system together with 5iLAF nave induction strategy, we performed integrative analysis across the timeline from human fibroblasts to nave iPSCs, which reveals ordered expression waves of gene networks sharing signatures with embryonic development process from post-implantation to pre-implantation stages. We also observed a significant transient re-activation of transcripts with 8-cell-stage-like characteristics in late nave reprogramming stages. Moreover, combined quantitative proteomics analysis with transcriptional dynamics during nave pluripotency induction, we identified ALPPL2 as the specific surface marker for human nave pluripotency. Altogether, our study deepens the understanding of molecular mechanisms of human nave pluripotency. SOURCE: Shaorong Gao (gaoshaorong@tongji.edu.cn) - Gaolab Tongji University

Pluto Bioinformatics

GSE89056: Integrative analysis of reprogramming human fibroblast cells to nave pluripotency [RNA-seq]