Pluto Bioinformatics

GSE44775: Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Bulk RNA sequencing

The degradation of TNF mRNA is critical for restricting TNF synthesis and involves a constitutive decay element (CDE) in the 3 untranslated region of the mRNA. We demonstrate that the active CDE is a stem-loop RNA motif that is recognized by the RNA-binding protein Roquin. Deep sequencing of Roquin-associated mRNAs identified CDE-containing mRNAs as the primary targets of Roquin on a transcriptome-wide scale. SOURCE: Georg Stoecklin (g.stoecklin@dkfz.de) - German Cancer Research Center