Pluto Bioinformatics

GSE128193: Mouse wound healing [RNA-seq]

Bulk RNA sequencing

Purpose: To explore the changes of genes expression in different time points during mouse wound healing.; Two wounds tissue from one animal were pooled, three animals were used per time point. The wounds tissue was collected on day 0, 1, 3, 5, 7 after cutting. Skin was cleaned of muscle and fat tissue; total RNA was extracted using the RNeasy mini kits (Qiagen), purified using Direct-zol RNA MiniPrep kit (Zymo Research). Next-generation libraries were prepared using the VAHTS TM mRNA-seq V2 Library Prep Kit for Illumina (Vazyme, #NR601). RNA-seq libraries were run on an Illumina HiSeq X-Ten next-generation sequencer. Analysis of RNA-seq data was done using the DESeq package in R. SOURCE: Junji Xu (junji.xu@nih.gov) - NIH, NIDCR