Pluto Bioinformatics

GSE119007: Prostaglandin E2 inhibits pro-fibrotic function of human pulmonary fibroblasts by disrupting Ca2+-signaling

Bulk RNA sequencing

Purpose: Identification of gene expression in human lung fibroblasts, including analysis of impact of IPF (Idiopathic Pulmonary Fibrosis) as well as TGF stimulation, with particular emphasis on prostanoid receptors and related pathways.; Methods: Truseq stranded mRNA sequencing, via Illumina Hiseq 4000. Single reads, 75bp, ~25 million reads per sample. Quantification of gene expression in TPM using Kallisto, followed by Tximport. mRNA isolated via Qiagen RNeasy kit, with on-column DNase1 digestion.; Results: Quantification of gene expression in primary human lung fibroblasts, including quantification of prostanoid GPCRs. SOURCE: Paul,A,Insel UC San Diego