Rapamycin inhibits mTORC1 complex in a variety of cells. We demonstrated that hESCs are more sensitive to rapamycin treatment as compared to somatic cells (BJ Fibroblasts) and also certain cancer cells (KBM7 cells). To study the molecular mechanisms of this disparity, we performed a transcriptome analysis (RNAseq) of rapamycin-treated hESCs and human fibroblasts for two time points (2 and 4 days of treatment). SOURCE: Mordecai Peretz (motti.peretz@mail.huji.ac.il) -  Hebrew University of Jerusalem

Pluto Bioinformatics

GSE107965: Transcriptome analysis of human embryonic stem cells (hESCs) and human fibroblasts upon rapamycin