Xist lacking of XR-PID (B-repeat and part of C-repeat) can't  recruit the polycomb during X chromsome inactivation. The majoring of Xist-mediated chromosomal silencing wouldnt be achieved. We found that hnRNPK was the one of main contributors for binding B-repeat of Xist. When we tethered hnRNPK to Xist which lacks of XR-PID region, the silencing and polycomb recruitment are restored. SOURCE: Guifeng Wei (guifeng.wei@bioch.ox.ac.uk) - Brockdorff Lab University of Oxford

Pluto Bioinformatics

GSE103362: 4sU-seq analysis for tethering hnRNPK to Xist lacking of XR-PID