Although membrane-anchored PD-L1 has been well-studied for its engagement with PD-1 on T cells to evade anti-tumor immunity, whether PD-L1 regulate oncogenic signaling pathways in tumor cells remains elusive.  Methods: Total RNA from MDA-MB-231 wild type (WT) , MDA-MB-231 PD-L1 knock-out (KO), CT26 WT or CT26 Pd-l1 KO cells was purified using Qiagen RNeasy mini kit (Qiagen) according to the manufacturers instructions. Library preparation and sequenceing analysis were performed at the Molecular Genetics Core facility in Dana-Farber Cancer institute. RNA-seq data was aligned using STAR v2.5.4a.   Conclusions: Our study indicates that PD-L1 may regulate genes that are involved in immune response regulating pathways. SOURCE: Chen Chu (chen_chu@dfci.harvard.edu) -  Dana-Farber Cancer Institute

Pluto Bioinformatics

GSE134510 (mouse): RNA sequencing of PD-L1 knock out human MDA-MB231 cells and mouse CT26 cells