Pluto Bioinformatics

GSE143479: Identification of alcohol-induced transcriptional changes in human esophageal epithelial cells via RNA-Seq

Bulk RNA sequencing

The current study is focused on elucidating the effect of acute alcohol challenge on esophageal epithelial cells. We employed in vivo mouse models, ex-vivo 3D-organoids and 2D cell culture to answer this question. RNA-Seq was employed to identify acute alcohol-induced transcriptional changes in human esophageal epithelial cells in-vitro. Acute-alcohol promoted strong mitochondrial damage resulting in bioenergenitic imbalance in esophageal epithelial cells. Autophagy was activated potentially via the AMPK-mTORC1 pathway to promtoe the clearance of damaged mitochondria. SOURCE: Michael Gonzalez (gonzalezm2@email.chop.edu) - The Children's Hospital of Philadelphia