Pluto Bioinformatics

GSE145469: Co-inhibition of BET proteins and PI3K triggers mitochondrial apoptosis in rhabdomyosarcoma cells

Bulk RNA sequencing

Remodeling transcription by targeting bromodomain and extraterminal (BET) proteins has emerged as promising anticancer strategy. Here, we identify a novel synergistic interaction of the BET inhibitor JQ1 with the PI3K-specific inhibitor BYL719 to trigger mitochondrial apoptosis and to suppress tumor growth in models of rhabdomyosarcoma (RMS). RNA-Seq revealed that JQ1/BYL719 co-treatment shifts the overall balance of BCL-2 family gene expression towards apoptosis and upregulates expression of BMF, BCL2L11 (BIM) and PMAIP1 (NOXA) while downregulating BCL2L1 (BCL-xL). SOURCE: Christina Grimm (cgrimm6@uni-koeln.de) - University Clinic Cologne